Journal: Biophysical Journal

Article Title: Characterizing Cell Adhesion by Using Micropipette Aspiration

doi: 10.1016/j.bpj.2015.06.015

Figure Lengend Snippet: Setup: in-plane (A–C) and profile modes (D and E). (A) Experimental setup for in-plane aspiration experiments. In this case, the micropipette is positioned perpendicular to the surface of adherent endothelial cells cultured on the bottom of a petri dish. The syringe pump on the left creates a constant-rate aspiration pressure increase. (B) Plot of the projected cell area versus time for three different cells. The projected cell area is measured from pictures taken throughout the detachment. (C) Time-lapse of a cell throughout a detachment assay (corresponding to the solid line in B, and to the cell on the right in Movie S1). Scale bar, 10 μm. (D) Experimental setup for profile aspiration experiments. In this case, the aspiration micropipette is still positioned perpendicular to the surface of the endothelial cells, but they adhere to a Cytodex-3 bead, held in position by a second, larger micropipette (on the right). (E) Time lapse of the detachment assay of an endothelial cell adhering to a Cytodex-3 bead. Scale bar, 10 μm.

Article Snippet: The microscope was equipped with a motorized micromanipulator carrying a first micropipette holder at a 45° angle, and a manual three-axis stage linked to a UT-2 joint to orient a second micropipette holder (MP285 micromanipulator, Sutter Instruments, Novato, CA; IM-H1 micropipette holders and UT-2 joint, Narishige, Tokyo, Japan; three-axis stage, Thorlabs, Newton, NJ).

Techniques: Cell Culture

Journal: Biophysical Journal

Article Title: Characterizing Cell Adhesion by Using Micropipette Aspiration

doi: 10.1016/j.bpj.2015.06.015

Figure Lengend Snippet: Critical stress. (A) Plot of binned experimental data showing that the detachment force scales linearly with the initial projected cell area, Scell, implying that a critical stress, σ∗ = 1300 Pa ± 50 Pa, induces cell detachment. Error bars show the standard deviation. N = 335 experimental data points. (B) Critical aspiration pressure, ΔP∗, versus projected cell area for different micropipette diameters. ΔP∗ depends on the micropipette section, Spipette, used in the experiments (●: Spipette ∈ [17 μm2, 72 μm2], ▲: Spipette ∈ [78 μm2, 275 μm2], ▪: Spipette ∈ [314 μm2, 707 μm2]). Error bars show the standard deviation.

Article Snippet: The microscope was equipped with a motorized micromanipulator carrying a first micropipette holder at a 45° angle, and a manual three-axis stage linked to a UT-2 joint to orient a second micropipette holder (MP285 micromanipulator, Sutter Instruments, Novato, CA; IM-H1 micropipette holders and UT-2 joint, Narishige, Tokyo, Japan; three-axis stage, Thorlabs, Newton, NJ).

Techniques: Standard Deviation